QUANTIFOIL® Holey Carbon Film supports with circular holes
QUANTIFOIL® Holey Carbon supports are a flat film of carbon, 10-12 nm thick, on a cryo-electron microscopy (cryo-EM) grid. They provide a low-background sample support ideal for high-resolution cryo-EM data collection. The optimum grid for your project can be selected from a wide variety of hole shapes, sizes and spacings in combination with a range of base grid materials and mesh sizes. Grid options include standard square meshes as well as other arrangements and finder grid formats.
QUANTIFOIL® Holey Carbon Film supports with circular holes are used in a wide range of applications from materials science to molecular biology and biomedical imaging. The circular holes allow improved sample distribution and reduced background scatter due to the formation of thin, even layers of vitrified ice that support the particles of interest. Users can select from a wide range of holes depending on their sample characteristics and imaging modality:
Small holes for high-resolution SPA of protein complexes;
Larger holes and less carbon to maximise open viewing area for tomography and materials science;
More Carbon and fewer holes for on-grid cell growth.
The optimum base grid mesh and QUANTIFOIL® Holey Carbon Film support geometry for a particular project depends on many variables, and researchers will want to try several combinations when starting a new project. Some general guidelines when identifying the ideal film geometry for your experiment include:
Magnification
Higher magnifications require smaller holes, as the presence of some support film in the image will aid focusing. The power spectrum of the amorphous carbon is much stronger than that of ice, which allows Thon rings to be more easily seen.
As a rough guide:
Use 1.2 μm holes and smaller (R 1.2/1.3 and R 1/1) at x 50,000 or higher magnification. These magnifications are normally used for high-resolution data collection for biological specimens.
Approx. 2 μm holes (e.g. R 2/1 or R 2/2) for x 30,000 and x 40,000
5 μm or larger (e.g. R 8/3 or R 6/6.5) at x 20,000
Sample
A larger sample size will require larger hole sizes. For example:
Proteins and protein complexes at high-resolution: R 1.2/1.3 or R 0.6/1
Viruses and tomography: R 2/1, R 2/2, R 2/4
Fibrils and other extended structures: R 3.5/1
Whole cells often need additional support, with holes more widely spaced: R 2/2, R 2/4, R 1/4, R 1.2/20
Application
Tomographic applications often benefit from larger open viewing areas due to tilting of the grid, so select smaller intervals between holes such as R 3.5/1
To increase data collection speed using beam-image shift, choose a larger hole size (R 2/1 or R 2/2), to allow multiple shots in the same hole before shifting the stage and re-focusing.
Quantifoil® Type | Hole Size | Spacing | Period |
R 0.6/1 | 0.6 | 1.0 | 1.6 |
R 1/2 | 1.0 | 2.0 | 3.0 |
R 1/4 | 1.0 | 4.0 | 5.0 |
R 1.2/1.3 | 1.2 | 1.3 | 2.5 |
R 1.2/20 | 1.2 | 20.0 | 21.2 |
R 2/1 | 2.0 | 1.0 | 3.0 |
R 2/2 | 2.0 | 2.0 | 4.0 |
R 2/4 | 2.0 | 4.0 | 6.0 |
R 3/3 | 3.0 | 3.0 | 6.0 |
R 3/5 | 3.0 | 5.0 | 8.0 |
R 3.5/1 | 3.5 | 1.0 | 4.5 |
R 5/10 | 5.0 | 10.0 | 15.0 |
R 5/20 | 5.0 | 20.0 | 25.0 |
R 6/6.5 | 6.0 | 6.5 | 12.5 |
R 6/100 | 6.0 | 100.0 | 106.0 |
R 10/5 | 10.0 | 5.0 | 15.0 |
R 10/10 | 10.0 | 10.0 | 20.0 |
R 10/20 | 10.0 | 20.0 | 30.0 |
R 17/5 | 17.5 | 5.0 | 22.5 |
Prod# |
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BQR0.6/1-2C |
Description |
---|
Quantifoil® R 1.6/1, 200 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1420.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
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BQR0.6/1-3C |
Description |
---|
Quantifoil® R 1.6/1, 300 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1420.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR0.6/1-4C |
Description |
---|
Quantifoil® R 1.6/1, 400 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1420.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR0.6/1-2A |
Description |
---|
Quantifoil® R 1.6/1, 200 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$2140.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
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BQR0.6/1-3A |
Description |
---|
Quantifoil® R 1.6/1, 300 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$2140.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR0.6/1-4A |
Description |
---|
Quantifoil® R 1.6/1, 400 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$2140.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# | Description | Unit | Price | Order/Quote |
---|---|---|---|---|
BQR0.6/1-2C | Quantifoil® R 1.6/1, 200 mesh, Cu | 100/pack | $1420.00 | Qty: Add to cart |
BQR0.6/1-3C | Quantifoil® R 1.6/1, 300 mesh, Cu | 100/pack | $1420.00 | Qty: Add to cart |
BQR0.6/1-4C | Quantifoil® R 1.6/1, 400 mesh, Cu | 100/pack | $1420.00 | Qty: Add to cart |
BQR0.6/1-2A | Quantifoil® R 1.6/1, 200 mesh, Au | 100/pack | $2140.00 | Qty: Add to cart |
BQR0.6/1-3A | Quantifoil® R 1.6/1, 300 mesh, Au | 100/pack | $2140.00 | Qty: Add to cart |
BQR0.6/1-4A | Quantifoil® R 1.6/1, 400 mesh, Au | 100/pack | $2140.00 | Qty: Add to cart |
Prod# |
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BQR1.2/1.3-2C |
Description |
---|
Quantifoil® R 1.2/1.3, 200 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1020.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR1.2/1.3-3C |
Description |
---|
Quantifoil® R 1.2/1.3, 300 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1020.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR1.2/1.3-4C |
Description |
---|
Quantifoil® R 1.2/1.3, 400 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1020.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR1.2/1.3-2A |
Description |
---|
Quantifoil® R 1.2/1.3, 200 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$1720.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
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BQR1.2/1.3-3A |
Description |
---|
Quantifoil® R 1.2/1.3, 300 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$1720.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR1.2/1.3-4A |
Description |
---|
Quantifoil® R 1.2/1.3, 400 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$1720.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# | Description | Unit | Price | Order/Quote |
---|---|---|---|---|
BQR1.2/1.3-2C | Quantifoil® R 1.2/1.3, 200 mesh, Cu | 100/pack | $1020.00 | Qty: Add to cart |
BQR1.2/1.3-3C | Quantifoil® R 1.2/1.3, 300 mesh, Cu | 100/pack | $1020.00 | Qty: Add to cart |
BQR1.2/1.3-4C | Quantifoil® R 1.2/1.3, 400 mesh, Cu | 100/pack | $1020.00 | Qty: Add to cart |
BQR1.2/1.3-2A | Quantifoil® R 1.2/1.3, 200 mesh, Au | 100/pack | $1720.00 | Qty: Add to cart |
BQR1.2/1.3-3A | Quantifoil® R 1.2/1.3, 300 mesh, Au | 100/pack | $1720.00 | Qty: Add to cart |
BQR1.2/1.3-4A | Quantifoil® R 1.2/1.3, 400 mesh, Au | 100/pack | $1720.00 | Qty: Add to cart |
Prod# |
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BQR2/1-2C |
Description |
---|
Quantifoil® R 2/1, 200 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1120.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR2/1-3C |
Description |
---|
Quantifoil® R 2/1, 300 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1120.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR2/1-4C |
Description |
---|
Quantifoil® R 2/1, 400 mesh, Cu |
Unit |
---|
100/pack |
Price |
---|
$1120.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR2/1-2A |
Description |
---|
Quantifoil® R 2/1, 200 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$1800.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR2/1-3A |
Description |
---|
Quantifoil® R 2/1, 300 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$1800.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# |
---|
BQR2/1-4A |
Description |
---|
Quantifoil® R 2/1, 400 mesh, Au |
Unit |
---|
100/pack |
Price |
---|
$1800.00 |
Order/Quote |
---|
Qty: Add to cart |
Prod# | Description | Unit | Price | Order/Quote |
---|---|---|---|---|
BQR2/1-2C | Quantifoil® R 2/1, 200 mesh, Cu | 100/pack | $1120.00 | Qty: Add to cart |
BQR2/1-3C | Quantifoil® R 2/1, 300 mesh, Cu | 100/pack | $1120.00 | Qty: Add to cart |
BQR2/1-4C | Quantifoil® R 2/1, 400 mesh, Cu | 100/pack | $1120.00 | Qty: Add to cart |
BQR2/1-2A | Quantifoil® R 2/1, 200 mesh, Au | 100/pack | $1800.00 | Qty: Add to cart |
BQR2/1-3A | Quantifoil® R 2/1, 300 mesh, Au | 100/pack | $1800.00 | Qty: Add to cart |
BQR2/1-4A | Quantifoil® R 2/1, 400 mesh, Au | 100/pack | $1800.00 | Qty: Add to cart |
Q:How can I place an order?
A: We apologize for the inconvenience, but our website currently does not support online purchasing. We're working on upgrading our purchasing system and will have it available soon. In the meantime, please email your order to sale@emart.tech. Thank you for your understanding.
Q2: When will my order be shipped?
A2: The lead time for QUANTIFOIL® sample supports varies significantly, depending on your order. Please consult your quotation for an estimated lead time: we will contact you if there is a problem with your order that may cause a delay.